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libretexts.org
8.5: Restriction Enzymes
DNA can be cut by restriction endonucleases (RE). Endonucleases are enzymes that can hydrolyze the nucleic acid polymer by breaking the phosphodiester bond between the phosphate and the pentose on …
Jul 21, 2019
Restriction enzyme DNA Cutting
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14K views · 446 reactions | EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio | Facebook
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